Objective:

To assess the potential eye irritation or corrosion caused by a test substance using albino rabbits.

Procedure:

1.     Animal Selection:

o      Use healthy adult albino rabbits.

2.     Test Substance Application:

o      Administer a single dose of the test substance into the conjunctival sac of one eye of each rabbit.

o      The other eye serves as a control and remains untreated.

3.     Observations and Scoring:

o      Observe the treated eye at specific intervals (1 hour, 24 hours, 48 hours, and 72 hours).

o      Record signs of irritation or corrosion, including ulceration, corneal opacity, iris inflammation, and conjunctival inflammation.

o      Score the severity of each effect using a standardized scale (e.g., Draize scoring system).

4.     Result and Interpretation:

o      Based on observations and scores:

·  Non-irritant: If no significant effects are observed.

·  Irritant: If mild to moderate effects occur.

·  Corrosive: If severe effects are evident.

Observation:

1.       Ulceration: Ulceration refers to the formation of open sores or wounds on the eye surface. It can be a severe sign of eye irritation or corrosion.

2.       Opacity of Cornea: Corneal opacity indicates cloudiness or loss of transparency in the cornea, which covers the front part of the eye. It can impair vision and is often associated with eye damage.

3.       Inflammation of Iris: Inflammation of the iris (iritis) involves redness, pain, and sensitivity to light. It affects the coloured part of the eye and can be indicative of eye irritation.

4.     Inflammation of Conjunctiva: Conjunctivitis (inflammation of the conjunctiva) results in redness,    discharge, and discomfort. It is a common response to eye irritants.